Asbagh LA et al. - In a study to investigate the inhibitory effects of zoledronic acid (ZA) on tumor-related growth factor IL-6 in hormone resistant prostate cancer cell lines, it was found that ZA exhibited a time- and dose-dependent apoptotic effect on PC-3 and DU145 prostate cancer cell lines and this effect was associated with inhibited secretion of IL-6 in DU145 cell line Methods
PC-3 and DU145 cell lines were treated with different concentrations of ZA (1-100 μM) at various intervals (24-72 h.)
Cell viability was investigated by XTT assay and apoptotic effect was evaluated by cell death detection ELISA kit
Caspase 3/7 activity assay was performed to confirm apoptosis
IL-6 levels were measured by ELISA in the supernatant, and these data were also confirmed by IL-6 mRNA analysis using RT-PCR
Results
PC-3 and DU145 cell lines were sensitive to ZA-mediated cytotoxicity in a dose- and time-dependent manner
The apoptotic effect was significantly different among PC-3 and DU145 cells
IL-6 secretion was significantly lower in both cell lines, compared with the untreated control cells
Although the increased inhibition of IL-6 secretion was associated with increased apoptosis in DU145 cells, there was no similar association for PC-3 cell line
When compared with the untreated controls, the number of cDNA copies was significantly lower in the ZA-treated DU145 cell line at doses of 30 and 90 μM, suggesting a reduced expression of IL-6 mRNA.
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