Plasma from systemic lupus patients compromises cholesterol homeostasis: A potential mechanism linking autoimmunity to atherosclerotic cardiovascular disease
Reiss AB et al. – Study provides evidence for impaired cholesterol homeostasis in systemic lupus erythematosus (SLE) and of immune involvement in atherogenesis. Strategies to inhibit or reverse arterial cholesterol accumulation may benefit SLE patients.Methods- Aim was to determine whether lupus plasma impacts expression of cholesterol 27-hydroxylase, an anti-atherogenic cholesterol-degrading enzyme that promotes cellular cholesterol efflux
- THP-1 human monocytes and primary human aortic endothelial cells (HAEC) were used for this study
- THP-1 monocytes and HAEC were incubated in medium containing SLE patient plasma or healthy control human plasma (CHP)
Results- SLE plasma decreased 27-hydroxylase message in THP-1 monocytes by 47 ± 8%, and in HAEC by 51 ± 5.5%
- THP-1 macrophages were incubated in 25% lupus plasma or CHP and cholesterol-loaded (50 µg/ml acetylated low density lipoprotein)
- Lupus plasma more than doubled macrophage foam cell transformation
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