Omeprazole blocks eotaxin-3 expression by oesophageal squamous cells from patients with eosinophilic oesophagitis and GORD
Cheng E et al. – Oesophageal squamous cells from gastro–oesophageal reflux disease (GORD) and Eosinophilic oesophagitis (EoE) patients express similar levels of eotaxin–3 when stimulated by Th2 cytokines, and omeprazole blocks that eotaxin–3 expression. These findings suggest that PPIs might have eosinophil–reducing effects independent of effects on acid reflux and that response to PPIs might not distinguish EoE from GORD.Methods
- Using telomerase–immortalised and primary cultures of oesophageal squamous cells from GORD and EoE patients, the authors measured eotaxin–3 protein secretion stimulated by Th2 cytokines (interleukin (IL)–4 and IL–13).
- Eotaxin–3 promoter constructs were used to study transcriptional regulation.
- Cytokine–induced eotaxin–3 mRNA and protein expression were measured in the presence or absence of omeprazole.
- There were no significant differences between EoE and GORD primary cells in cytokine–stimulated eotaxin–3 protein secretion levels.
- In EoE and GORD cell lines, IL–4 and IL–13 activated the eotaxin–3 promoter, and significantly increased eotaxin–3 mRNA and protein expression.
- Omeprazole blocked the cytokine–stimulated increase in eotaxin–3 mRNA and protein expression in EoE and GORD cell lines.