Association of nicotinamide adenine dinucleotide phosphate oxidase p22phox gene 549C>T polymorphism with coronary artery disease Full Text
Chinese Medical Journal, 05/01/2012
Tong–tao L et al. – The mutant genotypes of the p22phox gene 549C>T polymorphism had a significant effect on the increased risk of coronary artery disease in this studied population.Methods
- Hospital–based case–control study was conducted with 297 CAD patients and 343 healthy persons as the control group.
- Polymerase chain reaction and pyrosequencing using PSQ 96 MA Pyrosequencer (Biotage AB) were used to detect the polymorphisms.
- Multiple Logistic regression model was used to adjust the potential confounders and to estimate odds ratio (OR) with 95% confidence intervals (CIs).
- The observed genotype frequencies of this polymorphism obeyed the Hardy–Weinberg equilibrium in both cases (P=0.439) and controls (P=0.668).
- The frequency of mutant genotypes (TT+CT) in cases (41.08%) was higher than that in controls (36.73%) with an OR=1.20 (95% CI=0.87–1.65).
- After the adjustment of the potential confounders, there was a significant association of the mutant genotypes with increased risk of CAD (OR=1.57, 95% CI=1.01–2.46, P=0.047).