Histamine release-neutralization assay for sera of patients with atopic dermatitis and/or cholinergic urticaria is useful to screen type I hypersensitivity against sweat antigens
Archives of Dermatological Research, 05/03/2012
Shindo H et al. – In the study, the authors developed an assay for sera to neutralize histamine–releasing activity of semi–purified sweat antigen. The semi–purified sweat antigen was pre–incubated with serially diluted sera for 30 min at 37 °C and was subjected to histamine–release activity.