Novel peptidoglycan-based diagnostic devices for detection of wound infection
Diagnostic Microbiology and Infectious Disease, 09/06/2011
Hasmann A et al. – Apart from lysozyme, proteases [i.e., gelatinase matrix metalloproteinase MMP–2 and MMP–9 and elastase] were detected in wound fluids (e.g., using Western blotting). When dosed in ratios typical for wounds, a slight synergistic effect was measured for peptidoglycan hydrolysis (i.e., dye release) between lysozyme and these proteases. Incubation of a double–layer system consisting of stained and nonstained peptidoglycan with infected wound fluids resulted in a color change from yellow to blue, thus allowing simple visual detection of wound infection.
