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See Latest ArticlesDetection and subtyping of influenza A virus based on a short oligonucleotide microarray
Diagnostic Microbiology and Infectious Disease, 10/20/09
Li X et al. – The authors report the design and characterization of a microarray with 46 short virus–specific oligonucleotides for detecting influenza A virus of 5 subtypes: H1N1, H1N2, H3N2, H5N1, and H9N2. A unique combination of 3 specific modifications was introduced into the microarray assay: (1) short probes of 19 to 27 nucleotides, (2) simple amplification of full–length hemagglutinin and neuraminidase cDNAs with universal primers, and (3) Klenow–mediated labeling and further amplification of the samples before hybridization. The assay correctly and specifically detected and subtyped 11 different influenza A isolates from human, avian, and swine species representing the 5 subtypes.
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