Infectious Dis. Medical News about Vesicular Stomatitis

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Ribose 2'-O Methylation of the Vesicular Stomatitis Virus mRNA Cap Precedes and Facilitates Subsequent Guanine-N-7 Methylation by the Large Polymerase Protein
Journal of Virology, 10/14/09

Rahmeh AA et al. – This study provides new mechanistic insights into the mRNA cap methylase activities of VSV L, demonstrates that 2'–O methylation precedes and facilitates subsequent G–N–7 methylation, and reveals an RNA sequence and length requirement for the two methylase activities. The authors propose a model of regulation of the activity of the C terminus of L protein in 2'–O and G–N–7 methylation of the cap structure.

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