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See Latest ArticlesSpurious amplification of P. vivax small subunit RNA gene using the primers currently used to detect P. knowlesi
Journal of Clinical Microbiology, 10/08/09
Imwong M et al. – The PCR primers commonly used to detect P. knowlesi infections in humans were found to cross–react stochastically with P. vivax genomic DNA. A nested primer set that targets one the P. knowlesi small subunit ribosomal RNA genes, was validated for specificity and for sensitivity of detection of < 10 parasite genomes.
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