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Rapid SYBR Green I and modified probe real-time RT-PCR assays identify influenza H1N1 viruses and distinguish between pandemic and seasonal strains
Journal of Clinical Microbiology, 09/21/09
Yang JR et al. – A rapid SYBR Green I real–time RT–PCR assay was developed to identify pandemic H1N1 virus from clinical specimens in less than one hour. Probe real–time RT–PCR influenza A/B, H1/H3 and swNP/swHA assays were modified into the same PCR program, which allows rapid and simultaneous typing and subtyping of influenza viruses.
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