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Castro R et al. - The subtyping technique used in this study seems to have a good reproducibility. To authors' knowledge, subtype 10a was identified for the first time. Further studies are needed to explain the presence of this subtype in Portugal, namely its relationship to Treponema pallidum strains circulating in the African Portuguese speaking countries.

Exclusive Author Commentary
R Castro, 06/08/09

Monitoring the prevalence and the distribution of T. pallidum strains in specific geographic areas will allow us to characterize changes over time and the introduction of different strains in outbreaks. Since a sustainable in vitro culture system for T. pallidum is not possible, the availability of a reproducible typing system and confirmation of its reproducibility is of outmost importance.

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