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Boggild AK et al. - Nelson primer PCR demonstrated a single-organism level of analytic sensitivity. Performance characteristics of the assays varied by specimen type, with contact lenses and casings showing the highest rates of detectable acanthamoebae and highest diagnostic sensitivities for direct smear, culture, and JDP primer PCR, though these results are based on small numbers and should be interpreted cautiously. These findings have important implications for clinicians collecting diagnostic specimens, and for diagnostic laboratories, especially in outbreak situations.

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