Oncology Medical News

Become a Member Today!
Register

Most Viewed Abstracts

1. Report Shows Shift in Starting Salaries for Physicians

2. Recommendations on the use of 18F-FDG PET in oncology

3. Taxane-based combinations as adjuvant chemotherapy of early breast cancer: a meta-analysis of randomized trials

4. Gene expression signatures, clinicopathological features, and individualized therapy in breast cancer

5. Sentinel node biopsy is important in mastectomy for ductal carcinoma in situ

Your Article Summary

See Latest Articles

(Click the title below to leave the MDLinx Network and go to the Journal's Website)

Correlation of GREB1 mRNA with protein expression in breast cancer: validation of a novel GREB1 monoclonal antibody
Breast Cancer Research and Treatment, 11/04/09

Hnatyszyn HJ et al. – These data indicate that GREB1 mRNA expression correlates well with protein expression. The novel monoclonal GREB1ab is specific for GREB1 protein. This antibody will serve as a tool for investigations focused on the expression, distribution, and function of GREB1 in normal breast and breast cancer tissues.

Methods

A hybridoma expressing a murine monoclonal antibody targeting a 119 amino acid peptide specific to human GREB1 was generated
GREB1ab detects a 216 kDa protein corresponding to GREB1 in estrogen receptor alpha (ER?+) breast cancer cells as well as ER?? breast cancer cells transduced with a GREB1 expression vector

Results

ER?+ cell lines were observed to express GREB1 while ER?? cell lines did not express detectable levels of the protein
Using breast cancer tissue whole sections, IHC with the GREB1ab identified protein expression in ER?+ breast cancer tissue as well as normal breast tissue, with little GREB1 expression in ER?? breast cancer tissue

<< Previous
Menin, a product of the MENI gene, binds to estrogen receptor to...

Next >>
Phase 1/2 study of lumiliximab combined with fludarabine,...