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DNA damage induced by cis- and carboplatin as indicator for in vitro sensitivity of ovarian carcinoma cells
BMC Cancer, 10/15/09

Unger FT et al. – The measurement of DNA damage induced by cis- and carboplatin is an accurate method to determine the in vitro chemosensitivity of ovarian cancer cells towards these cytostatics, because of its quickness, sensitivity and low cell number needed.

Methods

DNA damage induced by cis- and carboplatin in primary cells of ovarian carcinomas determined by alkaline comet assay
In parallel reduction of cell viability measured by fluorescein diacetate (FdA) hydrolysis assay

Results

While in comet assay isolated cells showed high degree of DNA damage after 24 h treatment
Cell viability revealed no cytotoxicity after that incubation time
Individual sensitivities to DNA damage of 12 tumor biopsies differed up to factor of about 3
DNA damage after 1 day treatment with cis- or carboplatin correlated well with cytotoxic effects after 7 day treatment (r = 0,942 for cisplatin r = 0.971 for carboplatin)
Correlation of DNA damage and cytotoxicity induced by adriamycin was low or did not exist for gemcitabin

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