Oncology Medical News about epithelial-mesenchymal transition, twist, hepatocellular carcinoma

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Twist expression promotes migration and invasion in hepatocellular carcinoma
BMC Cancer, 07/21/09

Matsuo N et al. - In a study to evaluate the expression of twist and its effect on cell migration in hepatocellular carcinoma (HCC), it was shown that twist induces migratory effect on HCC by causing epithelial-mesenchymal transition (EMT).

Methods

Twist expression was examined using immunohistochemistry in 20 tissue samples of HCC, and twist expression was assessed in HCC cell lines by RT-PCR and Western blot analysis.
Ectopic twist expression was created by introducing a twist construct in the twist-negative HCC cell lines.
Endogenous twist expression was blocked by twist siRNA in the twist-positive HCC cell lines.
EMT related markers, E-cadherin, Vimentin, and N-cadherin were studied by Western blot analysis.
Cell proliferation was measured by MTT assay, and cell migration was measured by in vitro wound healing assay.
Immunofluorescent vinculin staining was used to visualize focal adhesion.

Results

Strong and intermediate twist expression was detected in 7 of 20 tumor samples, and no significant twist expression was found in the tumor-free resection margins.
Twist expression was detected in HLE, HLF, and SK-Hep1 cells, but not in PLC/RPF/5, HepG2, and Huh7 cells.
Ectopic twist-expressing cells demonstrated enhanced cell motility, but twist expression did not affect cell proliferation.
Twist expression induced epithelial-mesenchymal transition together with related morphologic changes.
Focal adhesion contact was reduced significantly in ectopic twist-expressing cells.
Twist-siRNA-treated HLE, HLF, and SK-Hep1 cells demonstrated a reduction in cell migration by 50, 40 and 18%, respectively.

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