Orlow I et al. – DNA damage as measured by the comet assay is associated with development of multiple primary tumors in non–small-cell lung cancer (NSCLC) pts Methods
Study of constitutive and induced DNA damage, and repair capacity in pts 108 with multiple NSCLCs (cases) and comparison of results to those in 99 pts with a single NSCLC (controls)
Cases and controls matched by age, sex, and time since diagnosis
Assessment of DNA damage on peripheral blood lymphocytes by the comet assay before and after exposing cells to a tobacco-derived carcinogen
Tail moment and tail intensity used as measures to assess baseline damage, induced damage, and repair capacity
Results
Constitutive DNA damage, benzo(a)pyrene diol epoxide–induced damage, and repair after BPDE-induced damage were all significantly higher in cases vs controls
Regression analyses adjusted for potential confounders confirmed these results
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