Bektas N et al. - A ubiquitin-like molecule interferon-stimulated gene 15 (ISG15) may represent a novel breast tumour marker with prognostic significance and may be helpful in selecting patients and predicting response to the treatment of human breast cancer. Methods
Aim was to present a systematic characterisation of ISG15 expression in human BCa (n=910) and normal breast tissue (n=135) at mRNA and protein level
Semiquantitative RT-PCR, cDNA dot blot hybridisation and IHC, was used to study ISG15 expression
ISG15 protein expression was analysed in two independent cohorts on tissue microarrays (TMAs)
In addition, a collection of benign and malignant mammary cell lines (n=9) were also investigated
Results
ISG15 was overexpressed in breast carcinoma vs normal breast tissue, both at the RNA and protein level
Recurrence-free, event-free, and overall survival analyses showed a correlation between ISG15 overexpression and unfavourable prognosis
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