Kuo S-H et al. – Autocrine BAFF signal transduction pathways may contribute to HP-independent growth of gastric MALT lymphoma (DLBCL[MALT]) Methods
Study of the role of B-cell activating factor of TNF family (BAFF) in mediating BCL10 nuclear translocation and HP-independence of gastric DLBCL(MALT)
Immunohistochemical and immunoblot analysis to measure expression of BAFF, pAKT, BCL3, BCL10, and NF-{kappa}B
Electromobility shift assay to measure transactivity of NF-{kappa}B
Review of lymphoma samples from 26 pts with gastric DLBCL(MALT)
Results
Aberrant BAFF expression of 7 of 10 (70%) HP-independent and in 3 of 16 (18.8%) HP-dependent cases
BAFF overexpression associated with pAKT expression, and nuclear expression of BCL3, BCL10, and NF-{kappa}B
In B-cell lymphoma Pfeiffer cells, BAFF activated NF-{kappa}B and AKT; activated NF-{kappa}B upregulated BCL10; activated AKT caused formation of BCL10/BCL3 complexes that translocated to the nucleus
Inhibition of AKT by LY294002 blocked BCL10 nuclear translocation, NF-{kappa}B transactivity, and BAFF expression
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