Angst E et al. – Mononuclear cells protect pancreatic cancer cells from drug-induced apoptosis in vitro by interleukin-1&Beta–mediated expression of cyclooxygenase-2 and production of prostaglandins; the importance of tumor-host interactions in pancreatic cancers may be a basis for novel therapeutic approaches to sensitize pancreatic cancers to chemotherapeutic agents Methods
Study of the role of mononuclear cells in pancreatic cancer chemoresistance
Differentiation of human histiocytic lymphoma U937 cells into macrophage-like cells
Enzyme-linked immunosorbent assay to measure the effect of U937-conditioned medium on drug-induced pancreatic cancer cell apoptosis
Specific receptor antagonists and inhibitors to evaluate interleukin-1&Beta and cyclooxygenase-2 contributions
Determination of the importance of extracellular signal-regulated kinase (ERK1/2) pathway
Results
U937-conditioned culture medium protected pancreatic cancer cells from drug-induced apoptosis
Interleukin-1 receptor antagonist and cyclooxygenase-2 inhibitor abolished this protective effect
U937-conditioned medium and interleukin-1&Beta stimulated expression of cyclooxygenase-2 and prostaglandin E2 production in pancreatic cancer cells, mediated by ERK1/2 pathway activation
Transfection of pancreatic cancer cells with cyclooxygenase-2 increased resistance to drug-induced cell death
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