Bahk JY et al. - In a trial to study the extrahypothalamic GnRH and GnRH receptor in bladder cancer epithelia, and the role of GnRH on bladder cancer cell proliferation, it was shown that bladder epithelia produce GnRH and the GnRH receptor in both normal and malignant tissues, but GnRH does not induce proliferation or cell cycle change of bladder cancer cells Methods
Normal human bladder mucosa, human transitional bladder cancer tissue, and 4 bladder cancer cell lines were used
For culture media, normal or charcoal-stripped serum with exogenous GnRH of different concentrations were supplemented
For detection of GnRH and the GnRH receptor and its mRNAs, RNase protection assay, in situ hybridization, and immunocytochemistry were performed
Cellular proliferation was measured by hemocytometer and the cell cycle by flow cytometer
Results
GnRH and GnRH receptor mRNA expression were detected in all cancer cell lines and human bladder cancer tissues
GnRH and GnRH receptors were localized in bladder cancer epithelial cells, but the density was not even
There were no significant differences in proliferation, and no significant change in cell cycle with GnRH supplements compared with controls
See all
