Lukasiak S et al. - In a study to confirm whether FAT10 is overexpressed in hepatocellular carcinoma (HCC) and in colon, ovary and uterus carcinomas, it was shown that FAT10 qualifies as a marker for an interferon response in HCC and colon carcinoma but is not significantly overexpressed in cancers lacking a proinflammatory environment Methods
FAT10 expression was tested in HCC, colon, ovary, and uterus carcinomas
Results
Elevated FAT10 expression in malignancies was attributed to transcriptional upregulation upon the loss of p53
FAT10 induced chromosome instability in long-term in vitro culture, which led to the hypothesis that FAT10 might be involved in carcinogenesis
Interferon (IFN)-γ and tumor necrosis factor (TNF)-α synergistically upregulated FAT10 expression in liver and colon cancer cells 10- to 100-fold
Real-time RT-PCR revealed that FAT10 mRNA was significantly overexpressed in 37 of 51 human HCC samples and in 8 of 15 human colon carcinomas
FAT10 cDNA sequences in HCC samples were not mutated and intact FAT10 protein was detectable
FAT10 expression in both cancer tissues correlated with expression of the IFN-γ- and TNF-α-dependent proteasome subunit LMP2 suggesting that proinflammatory cytokines caused joint overexpression of FAT10 and LMP2
NIH3T3 transformation assays revealed that FAT10 had no transforming capability
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