Song LL et al. - Notch-1 regulates nuclear factor-kappa B (NF-κB) in cervical cancer cells at least in part via cytoplasmic and nuclear IKK-mediated pathways. Methods
Cross talk between Notch-1 and NF-κB was studied in cervical cancer cells using standard laboratory techniques
Results
Wild-type, Notch-1 stimulates NF-κB activity in CaSki cervical cancer cells by associating with the IKK signalosome through IKKα
A significant fraction of TNF-α-stimulated IκB kinase activity in CaSki cells is Notch-1-dependent
Notch-1 is found in the nucleus in association with IKKα at IKKα-stimulated promoters
It is required for association of IKKα with these promoters under basal and TNF-α-stimulated conditions
Notch-1–IKKα complexes are found in normal human keratinocytes as well, suggesting that IKK regulation is a physiological function of Notch-1
Both Notch-1 and IKKα knockdown sensitize CaSki cells to cisplatin-induced apoptosis to equivalent extents
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