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Article Summary
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Garcia V et al. – VEGF mRNA marker may be similar to VEGF protein in plasma in colorectal cancer Methods- Study of VEGF mRNA in plasma as a possible surrogate marker of tumor angiogenesis in colorectal cancer pts
- Quantitative PCR to measure VEGF mRNA in plasma, tumors, and circulating tumor cells in colorectal cancer pts
- ELISA analysis of circulating VEGF protein
- Determination of microvessel density
Results- VEGF mRNA and plasma protein levels higher in pts vs controls
- VEGF mRNA overexpressed in tumors vs normal tissues
- VEGF protein levels associated with plasma VEGF mRNA; no associations with tumor samples
- High VEGF mRNA and vascular invasion showed trend to statistical significance
- MVD unrelated to plasma VEGF mRNA
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