Reda S Saad et al. – Molecular technology may be critical in differential diagnosis of dual primary carcinomas vs a metastatic breast cancer to contralateral breast; synchronous bilateral breast cancers represented two independent primaries rather than metastatic events in this study Methods
Molecular analysis of synchronous bilateral breast carcinoma to determine origin from a single or different clone
Review of 17 breast carcinoma pts: 12 pts with synchronous bilateral carcinomas and a control grp of 5 infiltrating ductal carcinomas with regional lymph node metastases
PCR to determine loss of heterozygosity (LOH) and microsatellite size alterations for a broad panel of 15 markers, involving 10 chromosomes
Classification of carcinomas as de novo or metastasis based on 3 concordance levels: marker-affected tumors concordant if >=50% of same markers were mutated; same gene copy affected; and temporal sequence of mutation acquisition
Results
Molecular analysis showed discordant mutations in all synchronous bilateral breast carcinoma cases, supporting the diagnosis of de novo bilateral primary breast carcinomas
Lymph node metastases pts had the same mutations in the primary breast carcinoma and metastases
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