Zheng H et al. - In a trial to investigate the pathobiological behaviors of gastric mixed-type (MT) carcinomas and gastric carcinogenesis, it was found that MT carcinomas were more aggressive than intestinal-type (IT) and diffuse-type (DT) carcinomas. Significant differences were observed in proliferation, apoptosis, angiogenesis, mucin secretion, and cell adhesion between IT and DT carcinomas, but only a few of these characteristics showed differences between MT intestinal and diffuse parts Methods
The expression of Ki-67, caspase-3, p53, fragile histine triad (FHIT), maspin, extracellular matrix metalloproteinase inducer (EMMPRIN), vascular growth factor (VEGF), MUC-2, 4, 5AC and 6, CD44, E-cadherin, β-catenin, and phosphorylated glycogen synthase kinase 3β-ser9 (P-GSK3β-ser9) was examined on tissue microarrays using immunohistochemistry
Results
MT carcinomas exhibited large size, deep invasion, frequent local invasion, and lymph node metastasis in comparison with IT and DT carcinomas
All the markers except MUC-5AC showed higher expression in IT than DT carcinomas
Expression of maspin, EMMPRIN, VEGF, MUC-4, and membrane E-cadherin was stronger in MT intestinal than diffuse component
Immunoreactivities to Ki-67, EMMPRIN, and VEGF were weaker in IT carcinoma than in the MT intestinal portion, while the opposite was true for CD44, MUC-2, and MUC-6
The MT diffuse component displayed higher expression of FHIT, VEGF, and P-GSK3β-ser9 than DT carcinoma
The accumulative survival rate of the IT carcinoma pts was higher than the other types
The invasive depth, venous invasion, lymph node, peritoneal or liver metastasis, and Lauren's classification were independent prognostic factors for gastric carcinomas
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