Yoo DG et al. - In a trial to investigate altered expression of apurinic/apyrimidinic endonuclease1/ref-1(APE1/ref-1) and antioxidant systems in lung cancer, the results suggest that APE1/ref-1 is up-regulated in the tumor regions of non-small cell lung cancer (NSCLC). Altered expression of antioxidant systems lead to enhanced production of superoxide production and lipid peroxidation, which can induce APE1/ref-1 in the tumor regions of NSCLC Methods
Tumor specimens from 48 pts with operable NSCLC were obtained
Immunohistochemistry, Western blot, lipid peroxidation, and superoxide production were performed on the tumor samples and a cultured H460 cell line
Results
APE1/ref-1 was mainly localized to the nucleus in the non-tumor regions of the lung cancer tissue specimens
Nuclear and cytoplasmic expressions of APE1/ref-1 in the lung cancers were markedly up-regulated in NSCLC specimens including squamous cell and adenocarcinoma specimens
Extracellular superoxide dismutase (ECSOD) and catalase were down-regulated and manganese superoxide dismutase (MnSOD) was up-regulated in the tumor regions of the NSCLC
Tumor regions of the NSCLC showed higher superoxide production and lipid peroxidation levels than non-tumor regions
In the lung adenocarcinoma cell line, H460, treatment with hydrogen peroxide in the presence of a catalase inhibitor, aminotriazole, increased APE1/ref-1 expression, suggesting oxidative stress might have contributed to the induction of APE1/ref-1
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