Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma
Jensen TO et al. - In a study to evaluate the prognostic role of soluble CD163 (sCD163) in serum and macrophage infiltration in primary melanomas from pts with American Joint Committee on Cancer (AJCC) stage I/II melanoma, it was shown that both serum levels of sCD163 and the presence of CD68+ macrophage infiltration at the tumor invasive front are independent predictors of survival in AJCC stage I/II melanoma. CD163+ cell infiltration in tumor stroma may be predictive of survival. Methods- Serum samples from 227 pts with stage I/II melanoma obtained before definitive surgery (baseline) and during 5 yrs of follow-up were analyzed for sCD163 by enzyme-linked immunosorbent assay.
- Excised formalin-fixed, paraffin-embedded primary melanomas from 190 pts were available for immunohistochemical analyzes of CD163+ and CD68+ macrophage infiltration.
- They were estimated semiquantitatively in 3 different tumor compartments: tumor nests, tumor stroma, and at the invasive front of the tumor.
Results- Serum sCD163 treated as an updated continuous covariate as well as the baseline value were analyzed together with the covariate's ulceration and thickness in a Cox proportional hazards model.
- sCD163 was an independent prognostic factor for overall survival (OS).
- Melanomas with dense CD163+ macrophage infiltration in tumor stroma and CD68+ macrophage infiltration at the invasive front were associated with poor OS independent of thickness and ulceration.
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