Quantification of PSA mRNA levels in peripheral blood of patients with localized prostate adenocarcinoma before, during, and after radical prostatectomy by quantitative real-time PCR (qRT-PCR)
Kalfazade N et al. - In a study to evaluate the clinical value of detected prostate-specific antigen (PSA) mRNA expression levels in circulating cells in pts with prostate cancer by qRT-PCR and determine the effect of surgical manipulation on hematogenous dissemination, it seems that highly detectable PSA mRNA expression levels in preoperative samples seem to be a significant predictable factor for prostate cancer recurrence Methods- Blood samples before, during, and after (24 h) surgery were obtained from 34 pts with prostate cancer who underwent radical prostatectomy for detecting PSA mRNA expression levels
- PSA mRNA expression levels, PSA levels, and Gleason scores were then compared
Results- PSA mRNA expression levels were detected in 14 of 34 pts, and biochemical recurrence of blood PSA was observed in 6 of 34 pts
- Differences of PSA mRNA expression levels in pre-, per-, and postoperative periods were statistically significant and differences of PSA mRNA expression levels in pre- and peroperative samples, pre- and postoperative samples, and per- and postoperative samples were also statistically meaningful
- There was significant relationship between the detectable PSA mRNA expression levels in preoperative samples and biochemical recurrence of blood PSA levels
- Surgical manipulation had no effect on biochemical recurrence of blood PSA
- There was significant correlation between high Gleason score and the detectable PSA mRNA expression levels
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