Overexpression of Pokemon in non-small cell lung cancer and foreshowing tumor biological behavior as well as clinical results
Zhao ZH et al. - In a study to investigate the expression of Pokemon in non-small cell lung cancer (NSCLC) and to explore its correlation with the clinical pathological characteristics and its influence on pts’ prognosis, there was high expression of Pokemon in NSCLC; Pokemon exerted carcinogenesis by inhibiting alternative reading frame (ARF), and had some clinical significance for prognostic evaluation of NSCLC pts Methods- Expression of Pokemon was determined in human NSCLC cell lines as well as 55 cases of NSCLC tumor tissues, tumor adjacent tissues, and surrounding tissues by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, and the relationship between Pokemon expression in NSCLC tumor tissues and clinicopathological features was analyzed
- Immunohistochemical techniques were used to determine 62 NSCLC tumor tissues (5 years ago) and p14ARF expression
- Correlation between them was observed and the effect of Pokemon on prognosis of pts with lung cancer was assessed
Results- Pokemon mRNA and protein took on high expression in lung cancer cell lines, and expression difference between cancer tissues, tumor adjacent tissues and surrounding tissues had statistical significance
- Pokemon expression and p14ARF expression were negatively correlated; expression of Pokemon was determined not to be associated with the pt's sex, age, smoking condition, tumor differentiation degree, histology and lymph node metastasis condition
- Its relationship with TNM staging was established
- Survival rate of pts with negative Pokemon expression was significantly higher than that of those with positive Pokemon expression
- Expression of Pokemon is believed to be an independent factor affecting prognosis
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