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Angiotensin converting enzyme DD genotype is associated with development of rheumatic heart disease in Egyptian children
Rheumatology International, 10/28/09

Morsy MMF et al. –This study was conducted to demonstrate this gene polymorphism in Egyptian children with RHD. Leukocytes DNA was extracted We concluded that patients with RHD have a higher ACE-DD genotype than normal control. ACE-DD genotype may be a risk factor for RHD in Egyptian children.

Methods

Leukocytes DNA extracted from 139 patients with RHD and 79 healthy control children
After amplification by PCR, the products were separated by electrophoresis in 6% polyacrylamide gel and visualized after ethidium bromide staining with UV light

Results

Gene polymorphism in both control and patients groups followed the following order of distribution from highest to lowest: ID, II, DD gene
Frequency in control group was 49.4, 36.7, and 13.9%, respectively
In patients groups, the gene frequency was 42.5, 30.9, and 26.6%, respectively
DD gene frequency differs significantly between the 2 groups

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