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A multiplatform real-time polymerase chain reaction detection assay for Vibrio cholerae
Diagnostic Microbiology and Infectious Disease, 10/22/09
Koskela KA et al. – The authors report a multiplatform real–time polymerase chain reaction methodology based on genes encoding for the regulatory toxR activator and enterotoxin A protein to determine enterotoxigenic Vibrio cholerae types from other vibrios. This assay, which was successfully validated on a collection of 87 bacterial strains, including 63 representatives of V. cholerae and 8 noncholera vibrios provides a rapid tool for detection and identification of cholera.
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