Microarray analysis of gastric mucosa among children with Helicobacter pylori infections
Pediatrics International, 03/23/2012
Ikuse T et al. – The authors analyzed the expression of immune factors using microarray and real–time polymerase chain reaction (PCR) to evaluate immune responses to H. pylori infection during childhood. They report that Lcn2, CCL18, CXCL9, CXCL11, PGI and PGII play important roles in childhood H. pylori infections.Methods
- Gastric samples were obtained from 12 patients undergoing routine endoscopic evaluation of chronic abdominal complaints.
- Six patients (3 boys and 3 girls) aged 10.1-14.6 years showed evidence of H. pylori infection, and the remaining six (3 boys and 3 girls) aged 10.3-15.5 years showed no evidence of infection and presented no histological changes associated with gastritis.
- Microarray and real-time PCR analyses were performed, and they analyzed changes in gene expression related immune response.
- Using microarray analysis, the total number of significantly up-regulated and down-regulated genes (fold change > 5, p < 0.01) was 21 in antrum and 16 in corpus when comparing patients with or without infection.
- Using real-time PCR, the expression of lipocalin-2 (Lcn2), C-C motif chemokine ligand (CCL) 18, C-X-C motif chemokine ligand (CXCL) 9 and CXCL11 was up-regulated, while the expression of pepsinogen (PG) I and PGII was down-regulated when comparing patients with or without infection.