Comparative analysis of inflamed and non-inflamed colon biopsies reveals strong proteomic inflammation profile in patients with ulcerative colitis Full Text
BMC Gastroenterology, 07/11/2012
Poulsen NA et al. – A distinct proteomic profile of inflamed tissue in UC patients was found. Specific proteins involved in energy metabolism and oxidative stress were identified as potential candidate markers for UC.Methods
- Biopsies were sampled from rectum, sigmoid colon and left colonic flexure from twenty patients with active proctosigmoiditis and from four healthy controls for proteomics and histology.
- Proteomic profiles of whole colonic biopsies were characterized using 2D–gel electrophoresis and peptide mass fingerprinting using matrix–assisted laser desorption/ionization time–of–flight mass spectrometry (MALDI–TOF MS) was applied for identification of differently expressed protein spots.
- A total of 597 spots were annotated by image analysis and 222 of these had a statistically different protein level between inflamed and non–inflamed tissue in the patient group.
- Principal component analysis clearly grouped non–inflamed samples separately from the inflamed samples indicating that the proteomic signature of colon mucosa with acute ulcerative colitis is strong.
- Totally, 43 individual protein spots were identified, including proteins involved in energy metabolism (triosephosphate isomerase, glycerol–3–phosphatedehydrogenase, alpha enolase and L–lactate dehydrogenase B–chain) and in oxidative stress (superoxide dismutase, thioredoxins and selenium binding protein).