Nuclear localization of 14-3-3epsilon inversely correlates with poor long-term survival of patients with colorectal cancer

Journal of Surgical Oncology, 04/20/2012

The current data provide evidence that 14–3–3ε is not exclusively a cytosolic protein, but is also detectable within the nucleus. The results suggest that nuclear 14–3–3ε as a suppressor may serve as important biomarker of tumor metastasis. Loss of nuclear 14–3–3ε is closely associated with poor overall survival in CRC patients.

Methods

  • Authors investigated 14–3–3ε expression and its prognostic significance in CRC. CRC surgical samples were taken from 137 clinicopathologically characterized CRC cases. 14–3–3ε expression was tested by immunohistochemical assay.
  • Separate Western blot of nuclear and cytosol preparations confirmed nuclear localization of 14–3–3ε protein.

Results

  • Nuclear expression of 14–3–3ε was observed in 76.9% of normal colorectal tissue and 78.8% of all CRC samples.
  • Statistical analysis showed that there was significant difference of nuclear 14–3–3ε expression in patients categorized according to lymph node metastasis.
  • A trend was identified between decreasing nuclear 14–3–3ε expression in CRC and worsening clinical prognosis.
  • Multivariate analysis showed that loss of nuclear 14–3–3ε expression was an independent prognostic indicator for patient's survival.

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