Fecal MMP-9: A new noninvasive differential diagnostic and activity marker in ulcerative colitis

Inflammatory Bowel Disease, 05/16/2012

The results highlight fecal metalloprotease 9 (MMP–9) as a useful tool in the differential diagnosis of diarrheic disorders and in the noninvasive evaluation of disease activity and mucosal healing in Ulcerative colitis (UC).

Methods

  • UC (n=47), IBS–D (n=23) patients, and control subjects (n=24) provided fecal samples for MMP–9 analysis.
  • In UC patients, disease severity was evaluated by the Mayo score.
  • Fecal MMP–9 and calprotectin levels were measured by enzyme–linked immunosorbent assay and lateral flow assay, respectively.

Results

  • MMP–9 was undetectable or ≤0.22 ng/mL in the feces of all controls and IBS–D patients.
  • In UC patients, fecal MMP–9 levels significantly correlated with the overall Mayo score (P < 0.001), the endoscopic score (P<0.001), and the serum C–reactive protein levels (P=0.002).
  • Additionally, in UC patients fecal MMP–9 levels showed a significant correlation with a known disease activity marker, fecal calprotectin (P = 0.014).

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