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Immunoassays for the incretin hormones GIP and GLP-1
Best Practice & Research Clinical Endocrinology & Metabolism, 09/21/09
Deacon CF et al. – The measurement of the incretin hormones, glucagon–like peptide–1 (GLP–1) and glucose–dependent insulinotropic polypeptide (GIP), using immunologically based assays is made difficult by the fact that the processing of the precursor molecules gives rise to a number of different peptides which cross–react with antisera raised against the two hormones. The use of highly specific assays using well–characterised antisera and careful sample handling is therefore required for a reliable determination of incretin hormone concentrations.
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