Almeida MQ et al. - IGF-IR overexpression was a biomarker of pediatric adrenocortical carcinomas. Additionally, a selective IGF-IR kinase inhibitor had anti-tumor effects in adult and pediatric adrenocortical tumor cell lines. Methods
Aim was to analyze expression of IGF-II and IGF-IR in pediatric and adult adrenocortical tumors
Effects of a selective IGF-IR kinase inhibitor (NVP-AEW541) on adrenocortical tumor cells was studied
57 adrenocortical tumors from 23 children and 34 adults
Gene expression was determined by QRT-PCR
Cell proliferation and apoptosis were analyzed in NCI H295 cells
A new cell line established from a pediatric adrenocortical adenoma was also used
Results
IGF-II transcripts were overexpressed in both pediatric adrenocortical carcinomas and adenomas
IGF-II was mainly overexpressed in adult adrenocortical carcinomas
IGF-IR expression was higher in pediatric adrenocortical carcinomas vs adenomas
Its expression was similar in adult adrenocortical carcinomas and adenomas
IGF-IR expression was a predictor of metastases in pediatric adrenocortical tumors
NVP-AEW541 blocked cell proliferation in a dose- and time-dependent manner in both cell lines through apoptosis
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