Abnormal expression and function of Dectin-1 receptor in type 2 diabetes mellitus patients with poor glycemic control (HbA1c>8%)
Metabolism, 05/17/2012
Clinical Article
Cortez–Espinosa N et al. – The data indicate that Dectin–1 may be involved in the abnormal immune responses that are observed in patients with T2D.
Methods- Dectin–1 expression was analyzed by flow cytometry and RT–PCR in monocytes and lymphocyte subpopulations from T2D patients (n=34) and healthy subjects (n=29).
- Functional assays were used to assess cytokine synthesis, ROS levels and oxidative stress ratio.
- The authors found increased expression (MFI) of Dectin–1 in monocytes from T2D patients.
- Significantly higher Dectin–1 expression was also detected in CD4+ T, CD8+ T, B cells and NK cells from T2D patients compared to controls.
- In contrast, monocytes from T2D patients with poor glycemic control (HbA1c>8%) showed a diminished percentage of Dectin–1+/TLR2+ cells.
- Negative correlations between the percent of Dectin–1+/TLR2+ cells and fasting plasma glucose levels (FPG) and HbA1c levels were found.
- A significant reduction in basal levels of IL–10 was observed in patients with poor glycemic control (HbA1c>8%) compared to patients with appropriate glycemic control (HbA1c≤6.5%) and healthy controls, an effect that was not observed in monocytes stimulated with zymosan.
- Higher ROS levels in zymosan–stimulated cells from patients with poor glycemic control positively correlated with FPG levels, and the oxidative stress ratio was higher in T2D cells compared with controls.
