Abnormal expression and function of Dectin-1 receptor in type 2 diabetes mellitus patients with poor glycemic control (HbA1c>8%)

Metabolism, 05/17/2012

Cortez–Espinosa N et al. – The data indicate that Dectin–1 may be involved in the abnormal immune responses that are observed in patients with T2D.

Methods

  • Dectin–1 expression was analyzed by flow cytometry and RT–PCR in monocytes and lymphocyte subpopulations from T2D patients (n=34) and healthy subjects (n=29).
  • Functional assays were used to assess cytokine synthesis, ROS levels and oxidative stress ratio.

Results

  • The authors found increased expression (MFI) of Dectin–1 in monocytes from T2D patients.
  • Significantly higher Dectin–1 expression was also detected in CD4+ T, CD8+ T, B cells and NK cells from T2D patients compared to controls.
  • In contrast, monocytes from T2D patients with poor glycemic control (HbA1c>8%) showed a diminished percentage of Dectin–1+/TLR2+ cells.
  • Negative correlations between the percent of Dectin–1+/TLR2+ cells and fasting plasma glucose levels (FPG) and HbA1c levels were found.
  • A significant reduction in basal levels of IL–10 was observed in patients with poor glycemic control (HbA1c>8%) compared to patients with appropriate glycemic control (HbA1c≤6.5%) and healthy controls, an effect that was not observed in monocytes stimulated with zymosan.
  • Higher ROS levels in zymosan–stimulated cells from patients with poor glycemic control positively correlated with FPG levels, and the oxidative stress ratio was higher in T2D cells compared with controls.

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