Glucocorticoid receptor {beta} and histone deacetylase 1 and 2 expression in the airways of severe asthma
Thorax,  Clinical Article

Butler CA et al. – Glucocorticoid receptor β (GR Β) mRNA is expressed at low levels in a minority of patients with severe asthma. histone deacetylase 2 (HDAC2) and HDAC2 expression was not downregulated in severe asthma. These data do not support upregulated GR β and resultant reduced HDAC expression as the principal mechanism of steroid resistance in severe asthma. Conflicting GR β literature may be explained in part by clathrin cross–reactivity with commercial antibodies.

Methods
  • Bronchoscopic study in two UK centres with real–time PCR and immunohistochemistry performed on biopsies, western blotting of bronchial epithelial cells and immunoprecipitation with anti–GR β antibody.
  • Protein and mRNA expression for GR α and HDAC2 did not differ between groups.

Results
  • GR Β mRNA was detected in only 13 of 73 samples (seven patients with severe asthma), however immunohistochemistry showed widespread epithelial staining in all groups.
  • Western blotting of bronchial epithelial cells with GR β antibody detected an additional ‘cross–reacting’ protein, identified as clathrin.
  • HDAC1 expression was increased in patients with severe asthma compared with healthy volunteers.

Please login or register to follow this author.
► Click here to access PubMed, Publisher and related articles...
<< Previous Article | Next Article >>

    Currently, there are no available articles.

Your Unread Messages in Emergency Medicine

See All >> Messages include industry-sponsored communications and special communications from MDLinx

Most Popular Emergency Medicine Articles

Indexed Journals in Emergency Medicine: Journal of Emergency Medicine, Annals of Emergency Medicine, Injurymore

Looking for a physician job? Let us help!

Register now for our free job placement service.
A career consultant will contact you soon.
Click Here for more information.

Get Started Now!

Free-of-charge, no obligation.

Terms and Conditions For M3 Career Consult

By registering with the M3 Career Consult service you authorize M3 USA Corporation to represent you in your job search. The following is a summary of the terms of our service and our mutual obligations to help you find a job:

  • You designate M3 to act as your agent and represent you in your job search;
  • You will not pay us for this service
  • You will advise us of any companies or positions to which you apply or have applied;
  • You will advise us if you believe you are not capable of performing a position for which we submit you, or for which you interview;
  • You will keep all information we provide you about companies or positions strictly confidential;
  • You will promptly provide us with information we request of you;
  • You will make yourself reasonably available for phone, video and in-person conversations and interviews;
  • You will be truthful in all of your communications with us; and
  • You will advise us if anything above changes.
 

The Top Read Articles of 2014 are Now Available

Scouring thousands of peer-reviewed journals and popular press, Our Editors just released the Top Read Articles of the Year. See the Top Read List