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Towards a ‘universal’ nucleant for protein crystallization
Trends in Biotechnology , 02/11/09

Saridakis E et al. - Protein structures are pivotal to the success of rational drug design and other biotechnology applications; however, obtaining high quality crystals poses a major problem to progress. Nucleation is the first step that determines the entire crystallization process, thus control of crystal nucleation would tackle the problem at its conception. A search for a ‘universal’ nucleant, a substance that can induce nucleation of any protein, is therefore on-going. The authors report the advances that have been made in this area, highlighting the success of especially engineered as well as naturally structured surfaces.

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