Allergy/Imm Medical News about T-SPOT.TB Spot

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Variation in T-SPOT.TB Spot Interpretation between Independent Observers from Different Laboratories
Clinical and Vaccine Immunology, 10/13/09

Franken WPJ et al. – The study was nested within an ongoing cohort study, in which part of the T–SPOT.TB had been performed with frozen material. Culture plates were read visually by four different observers from two laboratories and by two automated readers. Of 313 T–SPOT. The test result as positive or negative varied between independent raters, mainly due to samples with values around the cutoff. This warrants further study regarding determinants affecting the reading of T–SPOT.TB.assays, 235 were performed with fresh cells and 78 were performed with frozen cells.

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