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Single-Reaction Genomic Amplification Accelerates Sequencing and Vaccine Production for Classical and Swine Origin Human Influenza A Viruses
Journal of Virology, 09/11/09
Zhou B et al. – The authors used these procedures to rescue a contemporary H3N2 virus and a swine origin H1N1 virus directly from human swab specimens. Together, M–RTPCR and the modified reverse–genetics plasmids that we designed streamline the creation of vaccine seed stocks (9 to 12 days).
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