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Effects of macrophage colony-stimulating factor on microglial responses to lipopolysaccharide and beta amyloid
Cellular Immunology, 09/08/09
Vidyadaran S et al. – The authors show M–CSF expands primary microglia without affecting microglial responses to LPS and Abeta, as shown by the comparable expression of MHC class II and CD40 to microglia grown without this growth factor. M–CSF supplementation in BV–2 cells had no effect on nitric oxide (NO) production. Therefore, M–CSF can be considered for improving microglia yield in culture without introducing activation artefacts.
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