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Antineutrophil cytoplasmic antibody (ANCA) testing of routine sera varies in different laboratories but concordance is greater for cytoplasmic fluorescence (C-ANCA) and myeloperoxidase specificity (MPO-ANCA)
Journal of Immunological Methods, 07/28/09

Pollock W et al. – The 23 laboratories that responded to the questionnaire used 5 different IIF substrates and 11 ELISAs, and differed in other aspects of testing. Concordance for ANCA test results was greater for IIF–positive than an IIF–negative serum; for C–ANCA rather than P–ANCA; for MPO–ANCA rather than PR3–ANCA; and for strongly–positive rather than low positive PR3–ANCA. Concordance for test results might be improved with further standardisation of testing methodologies.

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