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See Latest ArticlesA fast and robust method to clone and functionally validate T-cell receptors
Journal of Immunological Methods, 06/29/09
Birkholz K et al. - The results of this assay correlated with the cytokine release, functional avidity, proliferative activity, and the ability to recognize MelanA/HLA-A2-presenting tumor cells of bulk T cells electroporated with RNA encoding the same TCR. Together these two protocols represent a rapid and low-cost tool for the identification and functional testing of TCRs of T-cell clones, which can then be applied in immunotherapy or immunological research.
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