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See Latest ArticlesCTLA4-Ig interacts with cultured synovial macrophages from rheumatoid arthritis patients and downregulates cytokine production
Arthritis Research & Therapy, 11/25/09
Cutolo M et al. – Optimal concentrations of CTLA4-Ig for the CTLA4-Ig/B7.2 masking on activated macrophages were identified and were found to induce significant downregulation in the cell production of IL-6, TNFalpha, IL1-beta and TGFbeta. In conclusion, macrophages would appear to be a sensitive target for CTLA4-Ig treatment in RA.
Methods- All macrophages co-cultured for 24 hours with activated T cells, without or with CTLA4-Ig (10, 100, 500 mg/ml for 1 hour, 3 hours and overnight, respectively)
- Immunofluorescence (IF) staining for B7.2, and analysis of inflammatory cytokine expression (interleukin(IL)-6, tumor necrosis factor(TNF)alfa, IL-1beta, transforming growth factor(TGF)beta) by immunocytochemistry (ICC), western blot (WB) and reverse transcriptase-polymerase chain reaction (RT-PCR) performed
- Macrophages showed intense B7.2 expression
- CTLA4-Ig/B7.2 masking evident for all macrophages, even after only 1 hour of cell culture (range from 10 to 100 mg/ml)
- ICC of co-cultures showed dose-dependent decrease in inflammatory cytokines (P<0.001 for IL-6, TNFalpha, IL-1beta and TGFbeta)
- Data confirmed by WB and RT-PCR analysis
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Today in Rheumatoid Arthritis...keeping you current
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Recent insights in the epidemiology of autoimmune diseases: Improved prevalence estimates and understanding of clustering of diseases
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