Pediatrics Medical News about MicroRNA, male breast cancer

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MicroRNA expression profiling of male breast cancer
Breast Cancer Research, 08/13/09

Fassan M et al. - In a trial to test the hypothesis that there is a specific miRNA expression signature that characterizes male breast cancers, it was concluded that specific miRNAs may be directly involved in male breast cancer development and they may represent a novel diagnostic tool in characterization of specific cancer gene targets.

Methods

Paraffin-blocks were obtained from 28 male pts including 23 breast cancers and 5 cases of male gynecomastia, and 10 female ductal breast carcinomas.
RNA harvested was hybridized to miRNA microarrays (~1100 miRNA probes, including 326 human and 249 mouse miRNA genes, spotted in duplicates).
To further support microarray data, an immunohistochemical analysis for 2 specific miRNA gene targets (HOXD10 and VEGF) was performed in a small series of male breast carcinoma and gynecomastia samples.

Results

A male breast cancer miRNA signature composed by a large portion of under-expressed miRNAs was identified.
17 miRNAs with increased expression and 26 with decreased expression were identified in male breast cancer compare with gynecomastia.
Among the miRNAs some had well characterized cancer development association and some showed de-regulation in cancer specimens similar to 1 previously observed in the published signatures of female breast cancer.
Comparing male to female breast cancers miRNA expression signatures, 17 significantly deregulated miRNAs were observed (4 over-expressed and 13 under-expressed in male breast cancers).
The HOXD10 and VEGF genes immunohistochemical expression significantly follows corresponding miRNA deregulation.